The identification of key genes for the internaliztion of peroxiredoixin protein by macrophage-like cell line

Msr1 and Mafb were identified as essential genes for the internalization of peroxiredoxin protein by this microarray analysis. We found that Msr1 and Mafb were deficient in mutant RAW lines. Overexpression of Msr1 or Mafb in mutant RAW cells regained the activity for the efficient internalization of peroxiredoxin protein. RAW cell internalized peroxiredoxin protein. Mutant RAW lines were generated by random mutagenesis with ENU treatment, which lost ability to internalize peroxiredoxin. We compared gene expression pattern between parental and mutant RAW cell line.

本研究通过微阵列分析（microarray analysis）鉴定出Msr1与Mafb是介导过氧化物还原酶蛋白（peroxiredoxin protein）内吞作用的关键基因。我们发现，突变型RAW细胞系中存在Msr1与Mafb的功能缺失。在该突变型RAW细胞中过表达Msr1或Mafb，可恢复其高效内吞过氧化物还原酶蛋白的能力。亲本RAW细胞能够内吞过氧化物还原酶蛋白。本研究通过N-乙基-N-亚硝基脲（ENU）随机诱变构建了丧失过氧化物还原酶蛋白内吞能力的突变型RAW细胞系。我们对亲本RAW细胞系与突变型RAW细胞系的基因表达谱进行了比较分析。