Sequencing analysis of immunopanned embryonic and early postnatal mouse RGCs. Sequencing analysis of immunopanned embryonic and early postnatal mouse RGCs

We report the genome-wide RNA sequencing changes to isolated retinal ganglion cells (RGCs) from immunopanned embryonic day 18 (E18) and early postnatal (P5) wildtype mouse retinas. We report the transcriptomic change associated with RGCs in a survival and regenerative state, and use gene-set enrichment analysis (GSEA) to predict the upstream transcription factors likely regulating these observed changes. Overall design: Time-course analysis of RGC transcriptomes in embryonic versus postnatal states.

本研究报道了从经免疫淘选的胚胎第18天（E18）与出生后早期（P5）野生型小鼠视网膜中分离得到的视网膜神经节细胞（retinal ganglion cells, RGCs）的全基因组RNA测序表达变化。本研究同时报道了处于存活与再生状态的RGCs相关转录组变化，并通过基因集富集分析（gene-set enrichment analysis, GSEA）预测了可能调控上述观测到的表达变化的上游转录因子。整体实验设计：针对胚胎期与出生后期RGC转录组的时间序列分析。