miRNA and consolidation of cued fear memory

miRNA profiling was carried out using the miRCURY LNA™ microRNA Array (6th gen - hsa, mmu & rno) miRNA were profiled in amygdala brain tissue obtained from adult mice 30 mins after auditory fear conditioning and expression levels compared to tissue obtained from Home cage controls Adult male mice were fear conditioned using tone-shock pairings and brains were harvested 30 mins later. The brains of Home Cage controls and Fear Conditioned animals (n = 4/group) were then punched to collect amygdala tissue. miRNA were extracted using the Qiagen miRNeasy Kit, and then shipped to Exiqon. Exiqon performed labeling, hybridization and data analysis after use of the miRCURY LNA™ microRNA Array (6th gen - hsa, mmu & rno). https://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA-microRNA-Array-6th-gen-hsa-mmu-rno-manual.pdf

本研究采用miRCURY LNA™微RNA芯片（第6代——覆盖人源（hsa）、小鼠（mmu）及大鼠（rno））开展微小核糖核酸（microRNA，以下简称miRNA）表达谱分析。实验针对成年小鼠经听觉恐惧条件化处理30分钟后的杏仁核脑组织进行miRNA表达谱检测，并与正常笼养对照组的脑组织样本进行表达水平对比。实验采用声音-电击配对范式对成年雄性小鼠实施恐惧条件化造模，于造模后30分钟处死并获取脑组织。随后对正常笼养对照组与恐惧条件化组小鼠（每组n=4）的脑组织进行穿刺取材以获取杏仁核组织。采用Qiagen miRNeasy试剂盒提取miRNA，随后将提取产物寄送至Exiqon公司。Exiqon公司使用前述miRCURY LNA™微RNA芯片（第6代——覆盖人源、小鼠及大鼠）完成样本标记、杂交及数据分析工作。相关芯片官方说明书可查阅：https://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA-microRNA-Array-6th-gen-hsa-mmu-rno-manual.pdf