Studies of wax genes in cotton (Gossypium arboreum)

The aim of the present study is to list the genes involved in cotton (G. arboreum) leaf epicuticular wax production and deposition. For this purpose differentially expressed genes (especially, down-regulated in wax deficient mutant plant) in wild and epicuticular wax mutant (Gawm3) plants were founded through cDNA microarray, developed from the wild plant leaves. 3× (wild cotton leaf labeled with Cy3/ epicuticular wax mutant cotton leaf (Gawm3) labeled with Cy5) 3× (wild cotton leaf labeled with Cy5/ epicuticular wax mutant cotton leaf (Gawm3) labeled with Cy3)

本研究旨在明确参与亚洲棉（G. arboreum）叶片表皮蜡质（epicuticular wax）合成与沉积的相关基因。为此，本研究以野生型植株叶片构建cDNA微阵列（cDNA microarray），通过该芯片检测野生型与表皮蜡质缺陷突变体（Gawm3）植株间的差异表达基因，重点关注在蜡质缺陷突变体中下调表达的基因。实验包含两组杂交重复：第一组为野生棉叶片标记Cy3荧光、表皮蜡质缺陷突变体（Gawm3）叶片标记Cy5荧光，共设置3次生物学重复；第二组为野生棉叶片标记Cy5荧光、表皮蜡质缺陷突变体（Gawm3）叶片标记Cy3荧光，同样设置3次生物学重复。