DNA Sequencing of Raw Milk Samples Collected from Holstein Cows Which Had K. pneumoniae Clinical Mastitis

16S rRNA gene amplicon sequencing was performed on DNA extracted from raw milk samples collected from Holstein cows which experienced a natural K. pneumoniae clinical mastitis infection. The main goal of this project was to understand the milk microbiome of cows before, during, and after, a K. pneumoniae mastitis infection. On select samples, shotgun metagenomic sequencing was performed as well.

本研究针对采集自患有自然发生肺炎克雷伯菌（Klebsiella pneumoniae）临床型乳房炎的荷斯坦奶牛的生牛乳样本，提取其基因组DNA并开展了16S rRNA基因扩增子测序（16S rRNA gene amplicon sequencing）。本项目的核心研究目标为解析奶牛在肺炎克雷伯菌乳房炎感染前、感染期间及感染后的牛乳微生物组组成。此外，针对部分选定的样本，本研究还开展了鸟枪法宏基因组测序（shotgun metagenomic sequencing）。