Gene expression changes in medulloblastoma due to Myc overexpression and Mitotic inhibition with AZD1152 Aurora Kinase B Inhibitior

Sonic hedgehog medulloblastoma cells with stable overexpression of Myc (UW426-Myc) become sensitized to apoptosis induction when exposed to Aurora kinase B inhibitor AZD1152. We sought to determine the gene expression changes that take place when Myc is overexpressed in UW426 and to determine the genes differentially expressed in UW426-Myc cells compared to wild-type UW426 when exposed tothe drug AZD1152 that specifically inhibits Aurora kinase B activity. Expression profiles were generated on the Illumina Human HT-12 v4.0 BeadChip platform for UW426-Myc and UW426 cells grown in standard culture media and exposed to no drug, 0.01% DMSO vehicle, or 100 nM AZD1152-HQPA for 48 hrs

本数据集针对稳定过表达Myc的音猬因子型髓母细胞瘤细胞（UW426-Myc）展开研究：该细胞在暴露于极光激酶B（Aurora kinase B）抑制剂AZD1152时，对凋亡诱导的敏感性显著提升。本研究旨在明确UW426细胞中Myc过表达时发生的基因表达变化，并对比野生型UW426细胞，确定暴露于特异性抑制极光激酶B活性的药物AZD1152后，UW426-Myc细胞中的差异表达基因。实验采用Illumina Human HT-12 v4.0 BeadChip芯片平台，对在标准培养基中培养的UW426及UW426-Myc细胞进行表达谱分析，处理条件包括无药物处理、0.01%二甲基亚砜（DMSO）溶剂对照，以及100 nM AZD1152-HQPA处理48小时。