Organ Scaffold Characterizations Using Intravital Microscopy Images

Abstract
This study contains intravital microscopy (IVM) data used to characterize whole organ scaffolds created using decellularization. Decellularization is a process that creates a cell-free collagen-based matrix from native organs, which can be used as scaffolds for regenerative medicine applications. This data set contains in vivoassays that evaluated the effectiveness of the decellularization process and structural and functional integrity of the acellular nephron in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, structural and functional integrities of various decellularized nephron segments, and velocity within the microcirculation were acquired and compared to the native (non-transplanted) organ. Cohorts of 2-3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n=4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). OIF, OIB, FV Multi-Tiff formatted files illustrate IVM measurement processes and are made publicly available in a data repository. Such data is intended to support scientific reproducibility, complement future renal studies, and extend the use of this powerful imaging application to analyze other organ scaffold systems.

摘要
本研究包含用于表征经脱细胞技术（decellularization）制备的全器官支架的活体显微镜（intravital microscopy, IVM）数据。脱细胞技术是一种从天然器官中制备无细胞胶原蛋白基基质的工艺，该基质可作为再生医学应用的支架材料。本数据集包含体内实验数据，用于评估脱细胞工艺的有效性，以及移植后环境中脱细胞肾单位的结构与功能完整性。研究人员对支架的DNA浓度、组织荧光信号、各脱细胞肾单位节段的结构与功能完整性，以及微循环内的血流速度进行了定性与定量评估，并将结果与天然（未移植）器官进行对比。实验使用2~3月龄的雄性斯普拉格·道利大鼠（Sprague Dawley rats）作为受试对象，分组如下：未移植组（n=4）、移植后第0天组（n=4）、移植后第1天组（n=4）、移植后第2天组（n=4）以及移植后第7天组（n=4）。以OIF、OIB、FV Multi-Tiff格式存储的文件记录了IVM成像的测量流程，已公开上传至数据仓库。本数据集旨在助力科研结果的可重复性验证，为未来的肾脏研究提供补充数据，并推动该先进成像技术在其他器官支架系统分析中的应用拓展。