bioreactor metagenome Genome sequencing. bioreactor metagenome

The metagenomic data were obtained from a study that operated bench-scale AeMBR and an AnMBR to investigate antimicrobial resistance patterns as a function of antibiotic loading and redox. Briefly, the AeMBR and the AnMBR were operated in parallel at 25 C and fed a synthetic feed representative of medium-strength domestic wastewater. Different antibiotic levels were applied in a stepwise fashion for a period of seven days at each of the concentrations (0, 10, and 250 ug/L). The sequencing data were then quality trimmed and filtered then assembled for further analysis.

本研究的宏基因组数据源自一项实验，该实验运行了实验室规模的好氧膜生物反应器（Aerobic Membrane Bioreactor, AeMBR）与厌氧膜生物反应器（Anaerobic Membrane Bioreactor, AnMBR），旨在探究抗菌耐药性模式随抗生素负荷与氧化还原条件的变化规律。简言之，两台反应器于25℃下平行运行，进水采用模拟中等强度生活污水的合成基质。实验以阶梯式施加不同浓度的抗生素，每个浓度水平（0、10、250 μg/L）均持续运行7天。后续对测序数据进行质量修剪与过滤，随后完成宏基因组组装以支撑后续分析。