Transcriptome data for the embryogenic callus formation in Bambusa changningensis

以 Bambusa changningensis Yi et B. X. Li 的芽作为外植体诱导愈伤组织形成。为分析不同发育阶段调控愈伤组织诱导的基因表达水平，我们从胚性愈伤组织 （EC） 与非胚性愈伤组织 （NE）、ECX vs NEX 和 ECP 与 NEP 中提取 mRNA 进行 Illumina 测序，共获得 62.08 Gb 的干净数据。每个样品包含 6.16 Gb 的干净数据，质量分数高于 30 （Q30） 的百分比超过 92.92%。与指定的参考基因组相比，通过对每个样本的干净读数进行测序，比对效率在 78.25% 至 81.36% 之间。ECX 与 NEX 共获得 4332 个 DEGs，其中 2846 个基因上调，1486 个基因下调。在 EC 与 NE 中发现了 4264 个 DEGs，其中 2408 个上调和 1856 个 DEGs下调。而在 ECP 和 NEP 中获得 7679 个 DEGs，后者有 4658 个上调基因和 3021 个下调基因。这些结果表明，NE 和 EC 不同发育阶段的基因表达水平存在显著差异。

Buds of Bambusa changningensis Yi et B. X. Li were used as explants to induce callus formation. To analyze the gene expression levels regulating callus induction at different developmental stages, we extracted mRNA from embryogenic callus (EC), non-embryogenic callus (NE), as well as the comparison groups ECX vs NEX and ECP vs NEP for Illumina sequencing, yielding a total of 62.08 Gb of clean data. Each sample generated 6.16 Gb of clean data, with the proportion of bases with a Phred quality score higher than 30 (Q30) exceeding 92.92%. By aligning the clean reads of each sample against the designated reference genome, the mapping efficiency ranged from 78.25% to 81.36%. A total of 4332 differentially expressed genes (DEGs) were identified in the ECX vs NEX comparison group, among which 2846 genes were up-regulated and 1486 were down-regulated. For the EC vs NE comparison, 4264 DEGs were identified, including 2408 up-regulated and 1856 down-regulated genes. For the ECP vs NEP comparison, 7679 DEGs were obtained, with 4658 up-regulated genes and 3021 down-regulated genes. These results indicate that there are significant differences in gene expression levels between non-embryogenic and embryogenic calli at different developmental stages.