Gene expression in HNSCC cell lines after knockdown of CTTN

The gene expression level of CTTN was decreased in HPV-negative HNSCC cell line with an amplification and overexpression of CTTN by performing knockdown experiments using RNA interference. Gene expression profiling and consecutive pathway analyses showed a number of deregulated genes known to be involved in metastatic processes such as migration and invasion. Furthermore, three potential downstream targets of CTTN (MAF, MAFB and PLAC2) were identified. cells with knockdown of CTTN vs. Control, 4 biological replicates

本研究利用RNA干扰（RNA interference）技术开展基因敲低实验，结果表明，在携带CTTN扩增与过表达的人乳头瘤病毒（HPV）阴性头颈部鳞状细胞癌（head and neck squamous cell carcinoma，HNSCC）细胞系中，CTTN的基因表达水平显著下调。随后通过基因表达谱分析与相继通路分析，本研究鉴定出一批已知参与迁移、侵袭等肿瘤转移过程的失调基因。此外，本研究还明确了CTTN的三个潜在下游靶基因：MAF、MAFB与PLAC2。本实验设置CTTN敲低组与对照组，每组设置4个生物学重复。