RNA-seq comprehensive analysis reveals the long non-coding RNA expression profile and co-expressed mRNA in adult degenerative scoliosis

Owing to the intensification of the aging process worldwide, the prevalence of adult degenerative scoliosis (ADS) is increasing at an alarming rate. However, genomic research related to the etiology of ADS is rarely reported worldwide. Since long non-coding RNAs (lncRNAs) play a pivotal role in the progression of human diseases, this study aimed to investigate ADS-associated messenger RNAs (mRNAs) and lncRNAs by RNA sequencing (RNA-seq), as well as performed comprehensive bioinformatics analysis. Overall design: Six whole blood (WB) samples were obtained from 3 ADS patients and 3 non-degenerative lumbar adult volunteers for RNA-seq exploration to construct differential mRNA and lncRNA expression profiles.

鉴于全球老龄化进程加剧，成人退行性脊柱侧凸（adult degenerative scoliosis，ADS）的患病率正以惊人速率攀升。然而，目前全球范围内针对ADS病因学的基因组研究仍鲜有报道。由于长链非编码RNA（long non-coding RNAs，lncRNAs）在人类疾病进展中发挥关键调控作用，本研究拟通过RNA测序（RNA-seq）技术，探究与ADS相关的信使RNA（messenger RNAs，mRNAs）及长链非编码RNA，并开展全面的生物信息学分析。整体实验设计：本研究纳入3名ADS患者与3名无退行性腰椎病变的成人志愿者，采集其共6份全血样本进行RNA测序，以此构建差异表达的mRNA及lncRNA表达谱。