Adapting Epigenetic Clocks for Cell-Free DNA High-Throughput Sequencing Data

We address the critical incompatibility of microarray-trained epigenetic clocks with sequencing data. Through systematic evaluation, we establish a best-practice framework to ensure accurate age estimation, unlocking the utility of sequencing assays for aging research and liquid biopsy applications本研究旨在解决经基因芯片（microarray）训练的表观遗传时钟（epigenetic clocks）与测序数据间存在的严重兼容性问题。通过系统性评估，我们构建了一套可保障年龄估算准确性的最佳实践框架，从而解锁测序检测在衰老研究与液体活检领域的应用潜力。