Isolation and characterization in a soil conditioned with foaming agents of a bacteria consortium able to degrade sodium lauryl ether sulphate. SLES degradation

bioaugmentation techniques. Enrichment cultures were performed using bacterial populations from spoil material collected in a tunnel construction site as the inoculum. A microbial pool able to grow in a few hours with SLES concentrations from 125 mg/L to 2 g/L was selected and then identified by NGS analysis. Most of the bacteria identified (99%) belonged to Gamma-Proteobacteria with Pseudomonas (ca 90%) the predominant genus. The microbial pool was able to completely degrade an initial SLES concentration of 250 mg/L in 9 hours. A predictive functional analysis using the PICRUSt2 software showed the presence of esterase enzymes, responsible for SLES degradation. The microbial pool selected could be useful for its possible seeding (bioaugmentation) on spoil material from tunneling excavation.

生物强化（bioaugmentation）技术相关研究：本研究以隧道施工现场采集的开挖弃土中的细菌群落作为接种物，开展富集培养实验。筛选得到一株可在125 mg/L至2 g/L的十二烷基醚硫酸钠（SLES）环境中于数小时内增殖的微生物菌群，随后通过下一代测序（NGS）技术完成菌种鉴定。鉴定结果显示，99%的已鉴定细菌隶属于γ-变形菌纲（Gamma-Proteobacteria），其中假单胞菌属（Pseudomonas）占比约90%，为该菌群的优势菌属。该微生物菌群可在9小时内将初始浓度为250 mg/L的SLES完全降解。利用PICRUSt2软件进行预测性功能分析后发现，菌群中存在负责催化SLES降解的酯酶类酶类。本次筛选获得的微生物菌群有望应用于隧道开挖弃土的生物强化接种处理。