A unique population of regulatory T cells in heart potentiates cardiac protection from myocardial ischemia/reperfusion injury

Purpose: The aims of the study were to identify that transctiptome of heart Tregs was different from lymphoid organ Tregs after I/R injury. Methods: Tregs from injured hearts and paired lymphoid organs of the Foxp3-GFP transgenic mice were double-sorted by magnetic-activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) to generate RNA-sequence 3 days after myocardial ischemia/reperfusion injury (I/R injury). Results: Transcriptional profiling revealed that the transcriptome of heart Tregs has unique characteristics when compared to lymphoid Tregs. mRNA profiles of injured heart Tregs, spleen Tregs and mediastinal lymph node (MLN) Tregs isolated from 8-week-old Foxp3-GFP mice 3 days after I/R injury.

研究目的：明确缺血再灌注（I/R）损伤后心脏调节性T细胞（Tregs）的转录组（transcriptome）与淋巴器官调节性T细胞的转录组存在差异。
方法：于心肌缺血再灌注（I/R）损伤3天后，从Foxp3-GFP转基因小鼠的受损心脏及配对淋巴器官中分离调节性T细胞，通过磁激活细胞分选（MACS）与荧光激活细胞分选（FACS）进行双分选，以开展RNA测序实验。
结果：转录组分析显示，与淋巴器官调节性T细胞相比，心脏调节性T细胞的转录组具有独特特征。本研究分析了缺血再灌注损伤3天后，从8周龄Foxp3-GFP转基因小鼠体内分离得到的受损心脏调节性T细胞、脾脏调节性T细胞及纵隔淋巴结（MLN）调节性T细胞的mRNA表达谱。