SARS-CoV-2 infection-induced human B cells. SARS-CoV-2 infection-induced human B cells

This study was designed to capture the single cell transcriptome and BCR sequence of B cells that were bait sorted using oligo-tagged viral antigens. Overall design: Human B cells (viable/CD19+/antigen-PE+ or viable/CD19+/antigen-APC+) were bait sorted after initial magnetic enrichment and used in downstream sequencing.

本研究旨在获取经寡核苷酸标记病毒抗原（oligo-tagged viral antigens）诱饵分选的B细胞的单细胞转录组（single cell transcriptome）与B细胞受体（BCR, B-cell receptor）序列。
整体实验设计如下：人类B细胞（活细胞/CD19+/抗原-PE+ 或 活细胞/CD19+/抗原-APC+）经初始磁珠富集后完成诱饵分选，所得细胞用于下游测序实验。