Transcriptomic profiling of circadian genes in WT mouse tissues (miRNA-Seq)

A goal of a collaboration between the CHDI Foundation and Prof. Jenny Morton at Cambridge University was to identify genes in mice with diurnal/circadian expression patterns. Tissues from wild type mice were harvested every 3 hrs over a 36 hour time period, for a total of 13 time points. Striatal, cortical, hypothalamic and liver samples were harvested, flash frozen, and transferred to Expression Analysis for RNA extraction and miRNA-seq sequencing. Samples passing QC were analyzed to produce high quality, 50-base paired-end RNA-seq results. Striatal, cortical, hypothalamic and liver samples were collected from 26-week-old wild type C57BL/6J male mice every 3 hours for 36 hours, number of replicates 2 to 12 for each ZT group. miRNA-Seq was performed.

本数据集由CHDI基金会（CHDI Foundation）与剑桥大学珍妮·莫顿（Jenny Morton）教授团队合作构建，其核心目标为鉴定具备昼夜节律（diurnal/circadian）表达模式的小鼠基因。研究人员以26周龄的野生型C57BL/6J雄性小鼠为实验对象，在36小时的周期内每3小时采集一次组织样本，共计设置13个时间点。采集的组织类型涵盖纹状体（Striatal）、皮层（Cortical）、下丘脑（Hypothalamic）与肝脏样本，样本采集后立即进行速冻（flash frozen）处理，随后被送至Expression Analysis平台开展RNA提取与小分子RNA测序（miRNA-seq）。通过质量控制（QC）的合格样本将用于后续分析，最终生成高质量的50碱基双端RNA测序（50-base paired-end RNA-seq）数据。每个节律时间（ZT）组的生物学重复数为2至12例，本数据集同时完成了小分子RNA测序（miRNA-Seq）。