RNA-Sequencing in Sulforaphane-treated Arabidopsis Seedlings

Ten-days old Col-0 seedlings grown in MS medium were sprayed with SFN in two different concentrations: 400 uM and 800 uM. DMSO (0.8% v/v) was used as a control. For each treatment, 3 biological replicates were carried out. The pool of complete seedlings (aerial part and root) was processed and RNA was extracted. The samples were sent to the RNAseq service with the ILLUMINA system (Exeter University Sequencing Services, UK). Funding was from BFF foundation.

将在MS培养基中培养的10日龄哥伦比亚生态型拟南芥（Col-0）幼苗，分别以400 μM和800 μM两种浓度的萝卜硫素（SFN）进行喷施处理；以体积分数0.8%（v/v）的二甲基亚砜（DMSO）作为空白对照。每组处理均设置3次生物学重复。收集完整幼苗（包含地上部分与根系）并混合后进行样本处理，随后提取总RNA。将样本送至英国埃克塞特大学测序服务中心，依托ILLUMINA测序系统开展RNA测序（RNAseq）服务。本研究受BFF基金会资助。