Arabidopsis thaliana Transcriptome or Gene expression. Arabidopsis thaliana

In this study, we systematically investigated the fate and phytotoxicity of carbon nanodots (C-dots, about 3 nm) in Arabidopsis thaliana (Arabidopsis), as well as the underlying potential mechanisms, by integrating transcriptomic, physiological and metabolomic techniques. The phytotoxicity of C-dots in Arabidopsis was concentration-dependent, C-dots at 125, 250, 500 and 1000 mg/L significantly reduced the root elongation by 25%, 40%, 56% and 61% compared with the control groups, but no significant inhibition were observed in 62.5 mg/L C-dots exposure. RNA-seq analyses showed that 76 and 715 genes in Arabidopsis roots while 112 and 636 genes in Arabidopsis shoots, which were involved in stimulus response, UDP-glycosyltransferase activity and cellular response to phosphate starvation, were up-regulated by 125 mg/L and 1000 mg/L C-dots, respectively. Meanwhile, 164 and 552 down-regulated genes in roots while 300 and 707 down-regulated genes in shoots were mainly involved in chloroplast structure and function.

本研究整合转录组学、生理学与代谢组学技术，系统探究了粒径约3 nm的碳纳米点（carbon nanodots，简称C-dots）在拟南芥（Arabidopsis thaliana，简称Arabidopsis）体内的归趋与植物毒性，及其潜在作用机制。C-dots对拟南芥的植物毒性呈浓度依赖性：与对照组相比，125、250、500及1000 mg/L的C-dots可显著抑制根伸长，抑制率分别为25%、40%、56%和61%；而62.5 mg/L的C-dots暴露未引发显著抑制效应。转录组测序分析显示，分别经125 mg/L和1000 mg/L C-dots处理后，拟南芥根部各有76个和715个基因、地上部分各有112个和636个基因发生上调，上述差异基因主要参与刺激响应、UDP-糖基转移酶（UDP-glycosyltransferase）活性调控以及细胞对磷饥饿的应答过程。与此同时，根部的164个、552个下调基因与地上部分的300个、707个下调基因，主要参与叶绿体结构与功能相关的生物学过程。