Hyperglycemic conditions induce rapid cell dysfunction-promoting transcriptional alterations in human aortic endothelial cells.. Hyperglycemic conditions induce rapid cell dysfunction-promoting transcriptional alterations in human aortic endothelial cells.

We report the first deep RNASeq time-series dataset (n=96 samples) of human aortic endothelial cells (HAECs) following exposure to normal (NG) and high glucose (HG) conditions over a time course from baseline to 24 hours (0, 0.5, 1, 4, 8 and 24 hours). We identify rapid and transient transcriptomic changes, alteration of molecular networks, and document temporal transcriptional dynamics of in HAECs. Overall design: RNA-seq time series analysis in HAECs under normal or high glucose conditions.

本研究报道了首个针对人大动脉内皮细胞（human aortic endothelial cells, HAECs）的深度RNA测序（RNA-seq）时序数据集，共包含96个样本。该数据集涵盖了细胞在正常葡萄糖（normal glucose, NG）与高葡萄糖（high glucose, HG）培养条件下，从基线至24小时（0、0.5、1、4、8及24小时）多个时间点的转录组测序数据。本研究鉴定出HAECs中快速且瞬时的转录组改变、分子网络重塑事件，并阐明了其随时间变化的转录动态特征。整体实验设计：在正常或高葡萄糖培养条件下，对HAECs开展RNA-seq时序分析。