High throughput sequencing of Siwi associated piRNAs from Bombyx mori BmN4 cells

RNAs associating with PIWI proteins were Immunoisolated from BmN4 cells. Sequence libraries were generated with NEBNext Small RNA Library Prep Set for Illumina(NEB). Libraries were sequenced using Illumina MiSeq (single-end, 51 cycles). Two Small RNA libraries were prepared from Flag-Siwi immuno-precipitate samples of BmN4 cells treated DDX43 siRNA

从BmN4细胞中免疫分离得到与PIWI蛋白结合的核糖核酸（Ribonucleic Acid, RNA）。采用适配Illumina测序平台的NEBNext小核糖核酸文库制备试剂盒（NEB）构建测序文库。使用Illumina MiSeq测序平台进行单端51个循环的测序。从经DDX43小干扰RNA（small interfering RNA, siRNA）处理的BmN4细胞的Flag-Siwi免疫沉淀样本中，制备了两个小核糖核酸文库。