AIB1D4 role in early breast cancer progression

We report the role of AIB1D4 in early stage breast cancer progression. Using CRISPR editing, we generated isogeneic cell lines that only express AIB1D4 and not full length AIB1 then extracted RNA for sequencing from cell lines and mamary fat pad xenografts in SCID/Beige mice. Data was aligned to Hg19 with STAR and processed with EdgeR. Examination of RNA-seq of 4 cell lines in triplicate and three pooled tumor xenografts (MCFDCIS alone, MCFDCIS-D4 and MCFDCIS + MCFDCIS-D4) in triplicate. UPDATE: [Jan-17-2025] The titles of Samples GSM4135857-GSM4135859 were swapped with the titles of Samples GSM4135860-GSM4135862.

本研究报道了AIB1D4在早期乳腺癌进展中的作用。本研究利用CRISPR编辑（CRISPR Editing）技术构建了仅表达AIB1D4而非全长AIB1的同基因细胞系（isogeneic cell lines），随后从该细胞系以及SCID/Beige小鼠的乳腺脂肪垫异种移植瘤中提取RNA用于测序。测序数据通过STAR比对软件（STAR）比对至人类参考基因组Hg19，并通过EdgeR分析工具（EdgeR）进行后续处理。本研究对4株细胞系的三份平行重复样品，以及3组混合肿瘤异种移植瘤（分别为单独MCFDCIS组、MCFDCIS-D4组以及MCFDCIS与MCFDCIS-D4共培养组）的三份平行重复样品开展了RNA测序（RNA-seq）分析。更新：[2025年1月17日] 样品GSM4135857至GSM4135859的标题与样品GSM4135860至GSM4135862的标题发生了互换。