Soil Fungal Community Responses to Long-term Nitrogen Addition at Cedar Creek

This project assesses the structure of soil fungal communities in a long-term N addition experiment at Cedar Creek Ecosystem Science Reserve, Minnesota, USA. Soil samples were taken in October 2018 from replicate plots with no, low, and high levels of nitrogen addition that have been fertilized continuously since 1983. Fungal communities were characterized with high-throughput sequencing of the ITS2 gene region using the primers in Taylor et al. (2017) Applied & Environmental Microbiology. Details about the experiment, including earlier fungal community responses, are available in Avis et al. (2003) New Phytologist.

本项目针对美国明尼苏达州雪松溪生态系统科学保护区的长期氮添加野外控制实验，系统评估土壤真菌群落结构。研究于2018年10月采集土壤样本，采样样地涵盖无氮、低氮与高氮三个氮添加梯度，所有样地自1983年起便持续施加氮肥并设置重复样方。真菌群落特征通过针对ITS2基因区域的高通量测序（high-throughput sequencing）进行解析，所用引物源自Taylor等人2017年发表于《应用与环境微生物学（Applied & Environmental Microbiology）》的研究成果。该实验的详细信息（含此前报道的真菌群落响应结果）可查阅Avis等人2003年发表于《新植物学家（New Phytologist）》的相关文献。