Expression data from WT CD24+CD172a+ cDC2s, WT CD24–CD172+ cDC2s, and WT CD24+CD172a– cDC1s

We used gene expression microarrays to determine if WT CD24+CD17a+ cDC2 are transcriptionally similar to WT CD172a+ cDC2 Splenic WT CD24+CD17a+ cDC2, WT CD24+ cDC1, and WT CD24-CD172a+ cDC2 were sorted for RNA extraction and hybridization on Affymetrix microarrays.

本研究采用基因表达微阵列技术，旨在探究野生型（Wild Type, WT）CD24⁺CD17a⁺经典树突状细胞2型（classical dendritic cell type 2, cDC2）与野生型CD172a⁺经典树突状细胞2型在转录水平上的相似性。实验过程中，我们分选了脾脏来源的野生型CD24⁺CD17a⁺cDC2、野生型CD24⁺经典树突状细胞1型（cDC1）以及野生型CD24⁻CD172a⁺cDC2，用于RNA提取及Affymetrix基因表达微阵列杂交。