k-mer matrix Aegilops tauschii diversity panel (Open wild wheat consortium phase) Part 3/3

Wild wheat relatives of bread wheat represent genetic diversity that can be used for wheat crop improvement. We generated a k-mer presence/absence matrix of over 920 accessions of the wild wheat Aegilops tauschii, the donor of the bread wheat D genome. This dataset was generated under the aegis of Phase II of the Open Wild Wheat Consortium (www.openwildwheat.org)., The full methods are available in the related publication., , # ***k***-mer matrix Aegilops tauschii diversity panel (Open Wild Wheat Consortium Phase II) PART 3/3

[https://doi.org/10.5061/dryad.wm37pvmvd](https://doi.org/10.5061/dryad.wm37pvmvd)

## **Description of the data and file structure**

*k*-mer presence/absence matrix for the *Aegilops tauschii* diversity panel of over 920 accessions, including genetically redundant accessions.

**Resequencing of the *Ae. tauschii* accessions:** We generated short-read whole genome sequencing data for 350 *Ae. tauschii* accessions.  PCR-free paired-end libraries were constructed and sequenced on an Illumina Novaseq 6000 instrument, yielding a median 8.3-fold coverage per sample (ranging from 5.87 to 16.86-fold).

***k*****-mer matrix generation.** We developed and implemented an optimised *k*-mer matrix workflow to generate a presence/absence *k*-mer matrix for large diversity panels (https://github.com/githubcbrc/KGWASMatrix). We counted *k*-mers (*k* = 51) in raw sequencing data for 350 accessions g...

面包小麦的野生近缘种蕴含可用于小麦作物改良的遗传多样性。我们生成了920余份野生小麦节节麦（Aegilops tauschii）材料的k-mer存在/缺失矩阵，节节麦是面包小麦D基因组的供体。该数据集是在开放野生小麦联盟（Open Wild Wheat Consortium）第二阶段的支持下生成的（网址：www.openwildwheat.org）。完整方法详见相关出版物。

# ***k***-mer矩阵 节节麦（Aegilops tauschii）多样性面板（开放野生小麦联盟第二阶段）第三部分/共三部分

[https://doi.org/10.5061/dryad.wm37pvmvd]

**数据与文件结构描述**

包含920余份材料（含遗传冗余材料）的节节麦（Aegilops tauschii）多样性面板的k-mer存在/缺失矩阵。

**节节麦材料重测序：** 我们对350份节节麦（*Ae. tauschii*）材料进行了短读长全基因组测序，生成测序数据。构建无PCR（PCR-free）双端文库，并利用Illumina Novaseq 6000仪器测序，每个样本的测序深度中位数为8.3倍（范围：5.87至16.86倍）。

**k-mer矩阵生成：** 我们开发并实现了优化的k-mer矩阵工作流程，用于生成大型多样性面板的k-mer存在/缺失矩阵（网址：https://github.com/githubcbrc/KGWASMatrix）。我们对350份材料的原始测序数据中的k-mer（k=51）进行计数，g...