Effect of H2O2 treatment on gene expression of islet derived hybrid cell of 1.1B4

We analyzed the comprehensive gene expression changes by H2O2 in pancreatic beta-cell derived 1.1B4 cells using Agilent microarray. The genes of 2903 H2O2 treated were up-regulated and 2283 genes were down-regulated compared with control sample. HMOX1, EGR1, IGF, IL8 and NR4A3 were strongly up-regulated Gene regulation associated with antioxidant defense system in pancreatic beta cells still needs to be elucidated, we analyzed the genes expression with Agilent micro-array after 4 hours of 100mM H2O2 treatment by one color method, and found 2903 genes were up-regulated and 2283 genes were down regulated. Changes of some genes expressions were confirmed by real time PCR.

本研究采用安捷伦微阵列（Agilent microarray）技术，分析过氧化氢（H₂O₂）处理胰腺β细胞来源的1.1B4细胞后的全面基因表达变化。结果显示，与对照样本相比，过氧化氢处理组共有2903个基因上调表达，2283个基因下调表达，其中HMOX1、EGR1、IGF、IL8及NR4A3的上调幅度最为显著。目前，胰腺β细胞中与抗氧化防御系统相关的基因调控机制仍有待阐明。为此，本研究采用单通道法，对经100mM过氧化氢处理4小时的上述细胞进行安捷伦微阵列基因表达分析，同样得到2903个基因上调、2283个基因下调的结果，并通过实时荧光定量PCR（real-time PCR）验证了部分基因的表达变化。