Data_Sheet_1_Screening and identification of the dominant antigens of the African swine fever virus.pdf

African swine fever is a highly lethal contagious disease of pigs for which there is no vaccine. Its causative agent African swine fever virus (ASFV) is a highly complex enveloped DNA virus encoding more than 150 open reading frames. The antigenicity of ASFV is still unclear at present. In this study, 35 proteins of ASFV were expressed by Escherichia coli, and ELISA was developed for the detection of antibodies against these proteins. p30, p54, and p22 were presented as the major antigens of ASFV, positively reacting with all five clinical ASFV-positive pig sera, and 10 pig sera experimentally infected by ASFV. Five proteins (pB475L, pC129R, pE199L, pE184L, and pK145R) reacted well with ASFV-positive sera. The p30 induced a rapid and strong antibody immune response during ASFV infection. These results will promote the development of subunit vaccines and serum diagnostic methods against ASFV.

非洲猪瘟（African swine fever）是一种致死率极高的猪传染性疾病，目前尚无有效疫苗。其病原体非洲猪瘟病毒（African swine fever virus, ASFV）是一种结构高度复杂的有包膜DNA病毒，编码超过150个开放阅读框（open reading frame）。当前，非洲猪瘟病毒的抗原性仍未明确。本研究利用大肠杆菌（Escherichia coli）表达了35种非洲猪瘟病毒蛋白，并建立了酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）以检测针对这些蛋白的抗体。研究结果显示，p30、p54与p22作为非洲猪瘟病毒的主要抗原，可与全部5份临床非洲猪瘟阳性猪血清以及10份实验感染非洲猪瘟的猪血清呈阳性反应。另有5种蛋白（pB475L、pC129R、pE199L、pE184L及pK145R）可与非洲猪瘟病毒阳性血清发生良好反应。p30在非洲猪瘟病毒感染过程中可诱导快速且强烈的抗体免疫应答。本研究结果将推动针对非洲猪瘟病毒的亚单位疫苗与血清学诊断方法的研发。