Sterilization of Mycobacterium tuberculosis Erdman Samples by Antimicrobial Fixation in a Biosafety Level 3 Laboratory

Incomplete sterilization of Mycobacterium tuberculosis Erdman cultures followed 1 h of incubation in low concentrations of glutaraldehyde (0.5 and 1.0%) or azide. In contrast, 2.5% glutaraldehyde, paraformaldehyde (2 or 4%), Vesphine IIse or 5% formalin sterilized these samples after 1 h. These results suggest caution in removing fixed M. tuberculosis samples from biosafety level 3.

将结核分枝杆菌Erdman菌株培养物置于低浓度戊二醛（glutaraldehyde，0.5%和1.0%）或叠氮化物（azide）中孵育1小时后，无法实现完全灭菌。与之相反，将该培养物置于2.5%戊二醛、2%或4%多聚甲醛（paraformaldehyde）、Vesphine IIse或5%福尔马林（formalin）中孵育1小时后，即可实现完全灭菌。上述结果提示，从生物安全三级（biosafety level 3）实验室移出固定化结核分枝杆菌样本时需格外谨慎。