A multiscale approach to decipher molecular mechanisms implicated in the intergenerational effect of ibuprofen on the mosquito Aedes aegypti. Molecular mechanisms implicated in the intergenerational effect of ibuprofen on the mosquito Aedes aegypti : focus on exposed female and non-exposed progeny (L1 larvae).

A laboratory strain of Ae. aegypti (Liverpool strain) was exposed to ibuprofen (1µg/L)from larvae hatching to imagoes emergence. To understand the mechanistic processes underlying the direct and intergenerational impacts of ibuprofen, we used RNA-seq based transcriptomic approach. Each condition (exposed or not to ibuprofen) was analyzed in triplicate, each RNA sample being extracted from 2-4 days old non-blood fed adult females (exposed or not) and from their progeny (L1 larvae exposed or not) . RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 150 bp single reads.

本研究使用埃及伊蚊（Ae. aegypti）利物浦品系的实验室种群，在其从幼虫孵化至成虫羽化的整个生命周期中，均暴露于浓度为1μg/L的布洛芬环境中。为解析布洛芬直接影响及代际影响背后的分子机制，我们采用了基于RNA测序（RNA-seq）的转录组学分析方法。每种处理组（布洛芬暴露组与未暴露对照组）均设置三次生物学重复；每份RNA样本分别取自2~4日龄未吸血的成年雌蚊（暴露组或对照组）及其子代（暴露或未暴露的L1期幼虫）。RNA测序文库在Illumina GAIIx测序仪上以150bp单端读长模式完成测序。