mRNA-seq of mus musculus: induced Th1 cells and Th17 cells

CD4+CD62L+ T cells isolated from splenocytes of C57BL/6N mouse. These cells were cultured in Th1 or Th17-polarizing condition in 96 well culture plate coated with anti-CD3e antibody and anti-CD28 antibody. In the differentiation period, the recombinant proteins which alter endogenous STAT1 were treated to compare the gene expression profile to non-treated group.

从C57BL/6N小鼠脾脏淋巴细胞中分离得到CD4+CD62L+ T细胞。将上述细胞接种至包被有抗CD3e抗体及抗CD28抗体的96孔培养板中，于Th1或Th17极化诱导条件下进行培养。在细胞分化阶段，使用可改变内源性STAT1的重组蛋白处理细胞，并与未处理组对比其基因表达谱。