Capability of FDA-EVIR Microarray for Detection of Norovirus and Hepatitis A Virus in Inoculated Tomatoes, Green Onions, and Celery. Capability of FDA-EVIR Microarray for Detection of Norovirus and Hepatitis A Virus in Inoculated Tomatoes, Green Onions, and Celery

The capability of the U.S. Food and Drug Administration Enteric Viruses tiling microarray (FDA-EVIR) was assessed for rapid molecular identification of human norovirus (NoV) and hepatitis A virus (HAV) extracted from artificially inoculated fresh produce. Two published viral extraction strategies, total RNA extraction or virus particle isolation, were employed to prepare the viral targets. We also assessed the amount of viral RNA extracted from celery by three commercially-available kits and how well that RNA performed on the FDA-EVIR. Our results confirm that FDA-EVIR can correctly identify common enteric viruses isolated from fresh produce and is capable of identifying single and mixed species of viruses, as well as distinguishing among genotypes. Extending microarray methods to other food matrices should provide important support to surveillance and outbreak investigations. Overall design: In total 17 samples; 11 Input files (different produces/viruses/extraction methods) with the corresponding 6 negative control samples

本研究评估了美国食品药品监督管理局肠道病毒铺瓦式微阵列（FDA-EVIR）用于快速分子鉴定人工接种新鲜农产品中提取的人类诺如病毒（NoV）与甲型肝炎病毒（HAV）的性能。本研究采用两种已发表的病毒提取策略——总RNA提取法或病毒颗粒分离法——制备病毒靶标。同时，本研究评估了三款商用试剂盒从芹菜中提取的病毒RNA的产量，以及该RNA在FDA-EVIR上的检测表现。研究结果证实，FDA-EVIR可准确鉴定从新鲜农产品中分离的常见肠道病毒，能够识别单一病毒、混合病毒物种，并可区分不同基因型。将该微阵列方法拓展至其他食品基质，可为病毒监测与暴发溯源调查提供重要支撑。实验整体设计：共包含17份样本，其中11份为输入样本（涵盖不同农产品、病毒种类及提取方法），配套6份阴性对照样本。