Space flight mouse plasma cell-free RNA seuqnencing in Mouse Habitat Unit-1 (MHU-1)

Purpose: Sequencing analysis of plasma cell-free RNA was performed to study the effects of space flight and microgravity environment in mice. Methods: Plasma samples were collected during the JAXA MHU-1 mission as reported previously (Shiba et al., 2017, Scientific Reports). Total RNA was purified from plasma samples and processed for sequencing analysis, to compare RNA expression profiles in ground control, space flight and artificial 1-G control groups. Plasma cell-free RNA profiles were compared among ground control, space flight and artificial 1G control groups.

研究目的：通过对小鼠血浆游离RNA（plasma cell-free RNA）进行测序分析，探究太空飞行与微重力环境对小鼠产生的影响。
研究方法：正如既往研究（Shiba等，2017年，《Scientific Reports》）所报道，血浆样本采集自日本宇宙航空研究开发机构（JAXA）的MHU-1任务。从血浆样本中纯化总RNA并开展测序分析，以对比地面对照组、太空飞行组与人工1-G对照组的RNA表达谱。本研究对地面对照组、太空飞行组及人工1-G对照组的血浆游离RNA表达谱进行了组间比较。