Distinct Intestinal Microbial Signatures Linked to Accelerated Biological Aging in People with HIV. Distinct Intestinal Microbial Signatures Linked to Accelerated Biological Aging in People with HIV

Genome-wide DNA methylation profiling of colon and ileal biopsies, blood samples from people living with HIV on ART and their matched HIV-negative counterparts. Despite having similar chronological ages, PWH on ART exhibit accelerated biological aging in the colon, ileum, and blood, as measured by various epigenetic aging clocks, compared to HIV-negative controls. Investigating the relationship between microbial translocation and biological aging, PWH on ART had decreased levels of tight junction proteins in the colon and ileum, along with increased microbial translocation. Overall design: Bisulfite converted DNA from PBMC, Colon, and Illeum tissue were hybridized to the Illumina MethylationEPIC Beadchip

本数据集针对接受抗逆转录病毒治疗（Antiretroviral Therapy, ART）的HIV感染者（people living with HIV, PWH）及其匹配的HIV阴性对照个体的结肠活检组织、回肠活检组织与血液样本，开展全基因组DNA甲基化谱分析。尽管两组人群的实足年龄相近，但与HIV阴性对照组相比，接受ART治疗的HIV感染者在结肠、回肠及血液样本中，经多种表观遗传衰老时钟（epigenetic aging clocks）检测后呈现出加速的生物衰老表型。为探究微生物易位（microbial translocation）与生物衰老之间的关联，本研究发现接受ART治疗的HIV感染者其结肠与回肠组织内的紧密连接蛋白水平出现下降，同时伴随微生物易位水平升高。实验整体设计：将来源于外周血单个核细胞（Peripheral Blood Mononuclear Cell, PBMC）、结肠组织与回肠组织的亚硫酸氢盐转化DNA，与Illumina MethylationEPIC BeadChip进行杂交实验。