Genome-wide maps of Runx3 bound regions in splenic NK cells. Mus musculus

ChIP-seq was conducted using freshly isolated (resting) splenic WT NK cells with anti-Runx3 antibody (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Overall design: Runx3 and H3K4me1 IP from splenic NK cells isolated by negative selection using NK cell isolation kit (R&D) followed by sorting of NKp46+ cells.

染色质免疫共沉淀测序（ChIP-seq）实验以新鲜分离的（静息状态）脾脏野生型NK细胞为实验材料，分别使用抗Runx3抗体（Ab）、抗H3K4me1抗体（Ab）以及作为对照的非免疫血清（NIS）开展实验。实验设计概述：采用R&D公司的NK细胞分离试剂盒通过阴性分选法分离脾脏NK细胞，随后分选出NKp46+阳性细胞，对其进行Runx3与H3K4me1的免疫沉淀（IP）实验。