Bulk RNA-seq on SEMA7A high and negative Idiopathic pulmonary fibrosis (IPF) lung fibroblasts

To check the gene signatures of SEMA7A high and SEMA7Anegative IPF lung fibroblasts and the similarity of SEMA7A high fibroblasts and invasive fibroblasts, SEMA7A negative fibroblasts and noninvasive fibroblasts, we sorted the SEMA7A high and negative IPF lung fibroblasts by flow cytometry and performed total RNA-seq. Define the gene expression profile of SEMA7A high and negative fibroblasts

为探究SEMA7A高表达与SEMA7A阴性特发性肺纤维化（Idiopathic Pulmonary Fibrosis, IPF）肺成纤维细胞的基因特征，以及SEMA7A高表达肺成纤维细胞与侵袭性肺成纤维细胞、SEMA7A阴性肺成纤维细胞与非侵袭性肺成纤维细胞之间的相似性，本研究通过流式细胞术分选SEMA7A高表达及阴性的IPF肺成纤维细胞，并开展全转录组RNA测序（total RNA-seq），以明确SEMA7A高表达与阴性肺成纤维细胞的基因表达谱。