microRNA-seq of TPR50 Tau P301S Transgenic Mouse in different brain regions and time-points

Purpose: The goal of this study was to assess miRNA expression changes in TPR50 Tau transgenic mice. The mice was backcrossed with 2 divergent mouse strains - DBA and FVB along with the parent C57BL6 strain. The resulting F1 hybrids were used for further analysis. Brain samples from F1 hybrids were isolated and falsh frozen. RNA was isolated using miRNeasy kit and RNA-seq was performed using standard Illumina kit for small RNA sequencing

研究目的：本研究旨在评估TPR50 Tau转基因小鼠的微小RNA（miRNA）表达变化。将该模型小鼠与DBA、FVB两个异源小鼠品系及亲本品系C57BL6进行回交，所得F1杂交子代用于后续分析。研究人员分离F1杂交子代的脑组织样本并实施快速冷冻。采用miRNeasy试剂盒提取RNA，并使用用于小RNA测序的标准Illumina试剂盒完成RNA测序。