Evaluation of Polymorphisms in IL8 and IL16 Genes in Patients with Acute Coronary Syndrome

Abstract Background: Acute coronary syndrome (ACS) is a cardiovascular disease caused by obstruction of coronary arteries by atheromatous plaque. Susceptibility to this disease may be related to genetic variations, such as single nucleotide polymorphisms (SNPs). Objective: In this study, we evaluated the relationship between SNPs in IL8 (rs4073; -251 A/T) and IL16 (rs11556218; T/G) genes and SCA in a Brazilian population. Materials and Methods: A sample of 200 patients with ACS and 50 non-ACS patients hospitalized at the Real Hospital Português, Recife - PE, Brazil, and 220 blood donors (donors) was used. Genotyping was carried out by polymerase chain reaction, and DNA sequencing. Statistical analyzes were performed using the Williams G, Chi-square and Kruskal Wallis tests, using the BioEstat 5.0 program, and the data with a value of p < 0.05 were considered significant. Results: In the IL8 gene, the AT genotype was the most frequent (p > 0.05) in all three groups. In the IL16 gene, genotypic distributions were different between patients with ACS and the donor group (p = 0.002), with the most frequent G allele in the second group (p = 0.0052). The IL-16 cytokine was higher in donors than in patients with ACS (p = 0.04) and the G (TG + GG) allele had higher values of this cytokine (p = 0.01). Conclusions: The results demonstrate the important role of the rs11556218 SNP in IL16 gene in SCA, evidencing that the G allele may be associated with a decreased risk of the disease.

摘要 背景：急性冠状动脉综合征（Acute Coronary Syndrome, ACS）是一种由动脉粥样硬化斑块阻塞冠状动脉引发的心血管疾病。该疾病的易感性可能与遗传变异相关，例如单核苷酸多态性（Single Nucleotide Polymorphism, SNPs）。目的：本研究旨在评估巴西人群中IL8基因（rs4073；-251 A/T）与IL16基因（rs11556218；T/G）的单核苷酸多态性与SCA的相关性。材料与方法：本研究纳入巴西伯南布哥州累西腓葡萄牙皇家医院（Real Hospital Português）收治的200例ACS患者、50例非ACS住院患者，以及220名献血者作为研究对象。采用聚合酶链式反应与DNA测序进行基因分型。统计分析采用Williams G检验、卡方检验及Kruskal-Wallis检验，使用BioEstat 5.0软件完成，以p<0.05为差异具有统计学意义。结果：IL8基因的AT基因型在三组人群中均最为常见（p>0.05）。IL16基因的基因型分布在ACS患者组与献血者组间存在显著差异（p=0.002），且G等位基因在献血者组中更为常见（p=0.0052）。IL-16细胞因子在献血者中的表达水平显著高于ACS患者（p=0.04）；携带G等位基因（TG+GG基因型）的个体其IL-16细胞因子水平更高（p=0.01）。结论：本研究结果证实了IL16基因rs11556218单核苷酸多态性在SCA发生中的重要作用，表明G等位基因可能与该疾病的发病风险降低相关。