Raw data "Fast and accurate quantification of double-strand breaks in microsatellites by digital PCR".

Figure 1: Southern blot original .tif images and quantification of the DSB in the xlsx files (see also Table 1). Figure 1BC-SB annotated.pptx represents the annotated Southern blots. Figure 2: Original image of the Ethidium bromide gel of the DSB-PCR. Image annotated with the main band around 500 bp and with indications of the TALEN ON (+DSB) or OFF (-DSB). Figure 3A: Original qPCR file (.pcrd) for the standard range. Ct results and calculations for the elaboration of the standard range are presented in the .xlsx files. Figure 3BC: Original qPCR files (.pcrd) for the SYBR Green concentration tests. Melting curves and Ct results for each SYBR Green concentration are presented in the .xlsx files. Figure 4CDE: Original dPCR files (.ncx and .ncr - can be opened using CrystalMiner software) for the quantification of the 31% DSB in the CTG repeats (quantified by Southern blot) and the 7% DSB (also quantified by Southern blot). Table 2: Sample 1 (31% DSB) and Sample 2 (7% DSB). xlsx files extracted from the dPCR .ncx file and represent the results obtained for DSB quantification (31% and 7% DSB). The extracted data provide results for the Blue_Channel_Concentration (cp/µL) and Green_Channel_Concentration (cp/µL), which allow us to calculate the percentage of DSB. Only the yellow lines in the xlsx files are used to quantify the DSB (representing 1 ng of DNA used in the dPCR reaction). Supplementary Data Figure 1A: Original qPCR file (.pcrd) for primer efficacy (CP19/upCTG fwd and JEM1 300bp primers used in this study). The xlsx file represents the quantification and calculations of primer efficacy. Supplementary Data Figure 1B: Original dPCR files (.ncx and .ncr - can be opened using CrystalMiner software) for the quantification of the number of positive droplets depending on DNA dilution to assess primer efficacy (CP19/upCTG fwd). The xlsx file represents the quantification and calculation of primer efficacy.图1：Southern印迹（Southern blot）原始.tif图像及xlsx文件中的双链断裂（double-strand break，DSB）定量结果（详见表1）。Figure 1BC-SB annotated.pptx为标注后的Southern印迹图。 图2：双链断裂PCR（DSB-PCR）溴化乙锭（Ethidium bromide）凝胶原始图像，已标注约500 bp主条带，以及TALEN开启（+DSB）与关闭（-DSB）的标记。 图3A：标准梯度的实时定量聚合酶链反应（quantitative real-time polymerase chain reaction，qPCR）原始文件（.pcrd格式）。用于构建标准梯度的Ct值结果及计算过程详见xlsx文件。 图3BC：SYBR Green浓度梯度测试的qPCR原始文件（.pcrd格式）。各SYBR Green浓度对应的熔解曲线及Ct值结果详见xlsx文件。 图4CDE：用于定量CTG重复序列中31% DSB（经Southern印迹定量）与7% DSB（亦经Southern印迹定量）的数字聚合酶链反应（digital PCR，dPCR）原始文件（.ncx与.ncr格式，可通过CrystalMiner软件打开）。 表2：样本1（31% DSB）与样本2（7% DSB）。xlsx文件由dPCR的.ncx文件提取得到，展示了DSB定量的结果（31%与7% DSB）。该提取数据包含蓝通道浓度（cp/µL）与绿通道浓度（cp/µL）的结果，可用于计算DSB百分比。仅使用xlsx文件中的黄色行进行DSB定量（对应dPCR反应中使用的1 ng DNA）。 补充数据图1A：用于检测引物效率的qPCR原始文件（.pcrd格式），本研究使用的引物为CP19/upCTG正向引物与JEM1 300bp引物。xlsx文件展示了引物效率的定量结果与计算过程。 补充数据图1B：用于通过DNA稀释梯度评估引物效率（CP19/upCTG正向引物）的dPCR原始文件（.ncx与.ncr格式，可通过CrystalMiner软件打开）。xlsx文件展示了引物效率的定量结果与计算过程。