Data_Sheet_1_Bacillus subtilis and Pichia farinose mixture improves growth performance and nutrient absorption capacity in broiler chicks.pdf

IntroductionThis study evaluated the effects of dietary supplementation of Bacillus subtilis and Pichia farinose mixture (BPM) on growth performance, apparent ileal digestibility, cecal bacteria counts, small intestinal morphology and digestive enzymes activities, and jejunal nutrient transporters gene expression in broiler chicks.
MethodsA total of 768 one-day-old Ross 308 broiler chicks were randomly Q18 assigned into 3 groups based on the initial body weight (42.00 ± 0.08 g). The experimental periods were 35 days. There were 16 replicates per group and 16 birds per cage. Dietary treatments included a basal diet supplemented with 0, 0.1, or 0.2% BPM to form CON, BPM0.1 (consisting Bacillus subtilis with 1.0 × 107 viable spore and Pichia farinose with 1.0 × 107 viable spore per kg diet), and BPM0.2 (consisting Bacillus subtilis with 2.0 × 107 viable spore and Pichia farinose with 2.0 × 107 viable spore per kg diet) groups.
Results and discussionDietary supplementation of graded levels of BPM has positive effects on growth performance of broiler chicks, manifesting in the increase of body weight gain during days 1–35 as well as the decrease of feed conversion ratio during days 1–7, 21–35, and 1–35. Moreover, BPM supplementation positively improved ileal energy and crude protein digestibility, increased Lactobacillus counts, optimized intestinal morphology, enhanced intestinal digestive enzymes activities, and upregulated jejunal SGLT-1, GLUT-2, and PEPT-1 expression. Therefore, BPM supplementation improved growth performance of broiler chicks, which was partially related to the improvement in intestinal nutrient absorption capacity.

引言
本研究评估了日粮添加枯草芽孢杆菌与粉状毕赤酵母复合混合物（BPM）对肉仔鸡生长性能、回肠表观消化率、盲肠菌群计数、小肠形态学与消化酶活性，以及空肠营养转运蛋白基因表达的影响。

方法
本试验共选用768只1日龄Ross 308肉仔鸡，按初始体重（42.00±0.08g）随机分为3组，试验周期为35天。每组设16个重复，每笼饲养16只肉仔鸡。日粮处理方案为在基础日粮中分别添加0、0.1%或0.2%的BPM，构建对照组（CON）、BPM0.1组（每千克日粮含枯草芽孢杆菌活菌芽孢1.0×10^7个与粉状毕赤酵母活菌芽孢1.0×10^7个）以及BPM0.2组（每千克日粮含枯草芽孢杆菌活菌芽孢2.0×10^7个与粉状毕赤酵母活菌芽孢2.0×10^7个）。

结果与讨论
日粮添加梯度剂量的BPM可对肉仔鸡生长性能产生积极影响，具体表现为1~35日龄体重增重增加，以及1~7日龄、21~35日龄和1~35日龄的料重比降低。此外，添加BPM可提升回肠能量与粗蛋白质表观消化率，增加盲肠乳酸杆菌数量，优化肠道形态结构，增强肠道消化酶活性，并上调空肠钠-葡萄糖协同转运蛋白1（SGLT-1）、葡萄糖转运蛋白2（GLUT-2）与肽转运蛋白1（PEPT-1）的基因表达水平。综上，日粮添加BPM可改善肉仔鸡生长性能，该效果与肠道营养吸收能力的提升存在部分关联。