Seawater carbonate chemistry and physiological data of a multi-driver aquarium experiment with different life stages of the cold-water coral Caryophyllia huinayensis

We conducted a six-month aquarium experiment with three life stages of Caryophyllia huinayensis to study their physiological response (survival, somatic growth, calcification and respiration) to the interactive effects of aragonite saturation (0.8 and 2.5), temperature (11 and 15 °C) and food availability (8 and 87 μg C/L). The fully crossed factorial design of this experiment with the three parameters resulted in eight treatments. We measured the calcification and respiration rates as well as the health status of the corals after one, three and six months. The survival rate was deteremined every month, the change in tissue covered surface area was measured between the beginning and end of the experiment and prey capture rates were determined after five months. During the whole experiment, the temperature was measured every 15 minutes with TidbiT temperature loggers and additional water parameters (temperature, salinity, pH and oxygen concentration) were measured daily. We took waters samples (total alkalinity and nutrients) once a week and used TA and pH (total scale) to calculate the carbonate chemistry using the programme CO2sys.

我们针对Caryophyllia huinayensis的三个生命阶段开展了为期六个月的水族箱实验，旨在研究其生理响应（生存、体细胞生长、钙化及呼吸）对文石饱和度（aragonite saturation，0.8与2.5）、温度（11℃与15℃）及食物可获得性（8与87μg C/L）交互作用的响应。本实验采用三参数完全交叉析因设计（fully crossed factorial design），共设置8个处理组。分别在实验进行1个月、3个月及6个月时，测定珊瑚的钙化速率、呼吸速率及健康状态。每月统计存活率；在实验开始与结束时测量组织覆盖表面积的变化；在实验进行5个月时测定猎物捕获率。整个实验期间，使用TidbiT温度记录仪每15分钟测量一次温度；每日测量其他水质参数（温度、盐度、pH值及氧浓度）。每周采集水样（测定总碱度（total alkalinity）及营养盐），并利用CO2sys程序，通过总碱度（TA）及总标度pH值计算碳酸盐化学参数。