Rate of ¹³C accumulation during a 0.5–10.0 min ¹³C_i-VHC pulse using 2% (w/w) ¹³C-NaHCO₃ and ¹³C enrichment at maximum labelling (20–60 min) in the pools of 3-phosphoglycerate (3PGA) and phosphoenolpyruvate (PEP).

The PEP/3PGA ratios were calculated from paired observations within each sample. Cultures were pre-acclimated and subjected to a chase of either 5% CO2 (HC) or 0.035% CO2 (LC) of ambient isotope composition. Note that compared to the HC condition, the LC condition resulted in faster and higher labelling of both the 3PGA and PEP pools. Also note that the PEP pool exhibited more rapid and higher labelling than the 3PGA pool. Each replicate experiment (WT n = 3, mutants n = 2) was an independent time series of 7–8 time points sampled ∼0.5–60.0 min after the pulse.磷酸烯醇式丙酮酸（PEP）与3-磷酸甘油酸（3PGA）的比值通过各样本内的配对观测值计算得到。培养物预先经过驯化，随后分别被置于环境同位素组成的5% CO₂（高碳组，HC）或0.035% CO₂（低碳组，LC）追踪培养条件下。需注意，相较于高碳组，低碳组的3PGA与PEP代谢池的标记速率更快、标记水平更高。同时需注意，PEP代谢池的标记速率与标记水平均高于3PGA代谢池。每组重复实验（野生型n=3，突变株n=2）均为独立时间序列，于脉冲标记后约0.5~60.0分钟内采集7~8个时间点的样本。