Nucleosome intermediate preference of SMARCAD1 chromatin remodeler, and the structural insights

Examination of the repositioning of nucleosome and hexasome-sized particles induced by the SMARCAD1 family proteins in cells support the hexasome-binding mode. Our findings reveal a new mode of chromatin regulation, wherein the nucleosome intermediates are specially remodeled through an ATP-dependent process. Overall design: MNase-seq in Saccharomyces cerevisiae. Compare nucleosome shift between WT-mutant(dFun30). Two biological replicates were sampled.

针对细胞内由SMARCAD1家族蛋白诱导产生的核小体（nucleosome）与六聚体尺寸颗粒的重定位现象开展的检测，佐证了六聚体结合模式。本研究结果揭示了一种全新的染色质调控模式：通过ATP依赖的过程对核小体中间态进行特异性重塑。实验设计：在酿酒酵母（Saccharomyces cerevisiae）中开展微球菌核酸酶测序（MNase-seq），对比野生型与dFun30突变型的核小体移位情况，共采集两份生物学重复样本。