George H. Patterson (2010) CIL:728, Chlorocebus sabaeus. CIL. Dataset

COS 7 cell expressing Organelle LightsTM PM-GFP (Invitrogen)consisting of the myristoylation/palmitoylation sequence from Lck
tyrosine kinase. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 1024 x 1024, pixel size is 0.1 µm x 0.1 µm and pixel time was set to 1.6 µsec. Detector pinhole was set to collect 29 ~0.5 µm slices at 0.5 µm intervals

COS 7 细胞表达 Organelle LightsTM PM-GFP（Invitrogen）的脂酰化序列，源自 Lck 酪氨酸激酶。图像采集采用蔡司 LSM510 META（Carl Zeiss, Thornwood, NY）搭载的 63X 1.4 NA Plan-Apochromat 油浸物镜，以 488 nm 激发光，HFT 488 二色滤光片和 LP505 发射滤光片进行。图像分辨率为 1024 x 1024，像素大小为 0.1 µm x 0.1 µm，像素时间设置为 1.6 µsec。探测器光阑设置为以 0.5 µm 间隔收集 29 个厚度为 0.5 µm 的切片。