Widely distributed neurotensinergic neurons in the brainstem regulate NREM sleep in mice

Microarray identifies enriched expression of Nts in SubLDT, compared to dorsal and ventral area Total RNA was obtained from brainstem tissues of 8-week-old male Chat-Cre: Ai9 mice, and applied for analysis.

基因微阵列（Microarray）分析结果表明，相较于背侧与腹侧区域，神经紧张素（Neurotensin, Nts）在亚背外侧被盖核（Sublaterodorsal tegmental nucleus, SubLDT）中存在富集表达。本实验提取了8周龄雄性Chat-Cre: Ai9小鼠的脑干组织总RNA，并将其用于本次分析。