Amplicon sequencing-based study of the diversity of Candidatus Hodgkinia cicadicola, endosymbiont of Magicicada septendecim

In many clades of cicadas (Insecta: Hemiptera: Cicadidae), including Magicicada septendecim, their bacterial nutritional endosymbiont Candidatus Hodgkinia cicadicola has evolved into complexes of genetically and cytologically distinct, interdependent lineages.We surveyed the Hodgkinia lineage diversity across M. septendecim broods. We did this by sequencing Hodgkinia protein-coding gene (etfD) amplicons from bacteriome tissue of different cicada individuals. The current BioProject groups BioSamples that correspond to different M. septendecim broods.在包括十七年蝉（Magicicada septendecim）在内的多个蝉类演化支（昆虫纲半翅目蝉科（Cicadidae））中，其细菌营养内共生体——候选哈金斯氏菌（Candidatus Hodgkinia cicadicola）已演化形成遗传与细胞学特征各异且相互依存的谱系复合体。本研究针对十七年蝉各蝉群中的哈金斯氏菌谱系多样性开展调研，通过对不同蝉个体菌胞组织内的哈金斯氏菌蛋白质编码基因etfD扩增子进行测序完成了该调研。当前的生物项目（BioProject）收纳了对应不同十七年蝉群的生物样本（BioSamples）。