Flow cytometry dataset of dexamethasone and immune checkpoint blockade in 4T1 breast cancer model (Myeloid panel)

Flow cytometry analyses were performed on cells isolated from tumors of 4T1-bearing mice to characterize myeloid cell populations. Cells were stained with Zombie Aqua for viability, FITC–I-A/I-E (MHC class II), PE–CD64, PE-CF594–Ly6G, PerCP-Cy5.5–CD11b, APC–CD103, PE/Cy7–Ly6C, and APC/Cy7–CD11c. This panel was designed to identify and quantify tumor-associated macrophages, monocytes, neutrophils, dendritic cell subsets, and related myeloid populations. These data provide insights into how dexamethasone, immune checkpoint blockade (ICB), or their combination influence the composition and activation status of myeloid cells in both the tumor microenvironment.本研究对4T1荷瘤小鼠肿瘤组织中分离得到的细胞开展流式细胞术（Flow Cytometry）分析，以表征髓系细胞群的特征。细胞经Zombie Aqua染色以评估细胞活力，同时使用FITC标记的I-A/I-E（主要组织相容性复合体II类，MHC class II）、PE标记的CD64、PE-CF594标记的Ly6G、PerCP-Cy5.5标记的CD11b、APC标记的CD103、PE/Cy7标记的Ly6C以及APC/Cy7标记的CD11c进行染色。该染色方案旨在鉴定并定量肿瘤相关巨噬细胞、单核细胞、中性粒细胞、树突状细胞亚群及相关髓系细胞群。本数据集揭示了地塞米松、免疫检查点阻断（ICB）或二者联合使用对肿瘤微环境中髓系细胞的组成与激活状态的影响。