BME/CTVM6 cells infected by SFTSV MOI 1

Study of tick cells from the Rhipicephalus microplus vector (BME/CTVM6) were infceted by SFTS virus at MOI of 1 PFU/cell. We collected samples at day 3 and 6 post-infection, and added the mock infected control. This was done in 4 replicates, and allow us to perform a proteomics informed by transcriptomic study, as well as differentially protein expression analysis.

本研究以微小扇头蜱（Rhipicephalus microplus）媒介来源的蜱细胞系（BME/CTVM6）为实验材料，以1 PFU/细胞的感染复数（Multiplicity of Infection, MOI）接种发热伴血小板减少综合征病毒（Severe Fever with Thrombocytopenia Syndrome Virus, SFTSV，简称SFTS病毒）。我们分别于感染后第3天和第6天采集样本，并设置模拟感染对照组。本实验共设置4次生物学重复，可为转录组学指导下的蛋白质组学研究以及差异蛋白质表达分析提供实验支撑。