Actively translating transcriptome (Ribotag) profiling of mouse retinal endothelial cells during postnatal development. Actively translating transcriptome (Ribotag) profiling of mouse retinal endothelial cells during postnatal development

Angiogenesis, a process mediating the expansion of vascular beds in many physiological and pathological settings, requires dynamic changes in endothelial cell (EC) behavior. The molecular mechanisms governing EC activity during different phases of vascular growth, remodeling, maturation, and quiescence remain elusive. Here, we have employed actively translating transcriptome analysis of mouse retinal ECs for the characterization of dynamic gene expression changes during postnatal development and the identification of critical angiogenic factors.

血管生成（Angiogenesis）是介导多种生理与病理状态下血管床扩张的核心过程，其依赖于内皮细胞（endothelial cell, EC）行为的动态改变。在血管生长、重塑、成熟及静息等不同阶段中，调控内皮细胞活性的分子机制仍未明晰。本研究针对小鼠视网膜内皮细胞开展主动翻译转录组分析，以此表征小鼠出生后发育过程中的动态基因表达变化，并鉴定关键血管生成因子。