Supplemental Material for Liu et al., 2020 (post accept)

Figure
S1 shows the sequence of the synthetized tdTomato gene with flanking homologies
to SIR2. Figure S2 illustrates the growth of clones with subtelomeric URA3
either on –ura plates or on 5-FOA plates. Figure S3 and S4 describe the
recovery of the initial expression profile after cell sorting for both low and
high Sir2-expressing cells, after 6 h and 24 h respectively. Figure S5 shows
the viability of subpopulations of the tdTomato-Tsl1-tagged strain expressing
extreme tdTomato levels similar than in the tdTomato-Sir2-tagged strain. Table
S1 lists the primers used in this study. Table S2 contains the raw data of the
silencing and viability analyses

图S1展示了合成的tdTomato基因序列，该基因携带有与SIR2同源的侧翼序列。图S2展示了携带端粒旁侧URA3基因的克隆在-URA培养基平板与5-氟乳清酸（5-FOA）平板上的生长情况。图S3与图S4分别展示了低表达Sir2的细胞在细胞分选后6小时、高表达Sir2的细胞在细胞分选后24小时时的初始表达谱恢复情况。图S5展示了带有tdTomato-Tsl1标签的菌株的各亚群存活率，该菌株的tdTomato表达水平与带有tdTomato-Sir2标签的菌株的极端表达水平相近。表S1列出了本研究中使用的引物。表S2收录了基因沉默与细胞存活率分析的原始数据。