Isolation and identification of metalotolerant bacteria with possible biotechnological application from two mining tails from the Sonoran River basin, Mexico. Microbacterium ginsengisoli

An enrichment and isolation media procedure were performed to obtain bacteria with lower nutritional requirements and high tolerant to Cupper and Iron from two different mining tails from the Sonoran River basin. Two different strains were isolated and identified from San Felipe de Jesus mining tail from Staphylococcus genus and one of them identified as Microbacterium ginsengisoli, On the other hand, three isolated strains for Nacozari de Garcia mining tail were found: Burkholderia cenocepacia, Sphingomonas sp. and Staphylococcus warneri. Our results reveal significant diversity differences between the two compared sites and report species not previously documented at each location. Importanly, all these species exhibited a tolerance up to 300 ppm for a Cu and Fe multimetal solution indicating their potential for biotechnological application. Subsequently, a consortium was formed with these isolated bacteria, and for a potential biotechnological application of these bacteria, they were immobilized in two biocomposites of pecan nutshell, with the larger pecan nutshell biocomposite achieving greater immobilization.

本研究采用富集分离培养基流程，从索诺兰河流域的两处不同尾矿中分离获取营养需求更低、对铜（Cupper）和铁具有高耐受性的细菌。从圣费利佩·德·赫苏斯（San Felipe de Jesus）尾矿中分离得到2株细菌，经鉴定均属于葡萄球菌属（Staphylococcus genus），其中1株经鉴定为人参微杆菌（Microbacterium ginsengisoli）；另一方面，从纳科扎里·德·加西亚（Nacozari de Garcia）尾矿中分离得到3株细菌，分别为洋葱伯克霍尔德菌（Burkholderia cenocepacia）、鞘氨醇单胞菌属（Sphingomonas sp.）以及沃纳葡萄球菌（Staphylococcus warneri）。本研究结果显示两处采样位点的菌群多样性存在显著差异，且报告了此前在两地未被记录的物种。重要的是，所有受试菌株在铜、铁复合金属溶液中均可耐受最高300 ppm的浓度，表明其具备生物技术应用潜力。随后，研究团队将上述分离得到的细菌构建为复合菌群；为实现该类细菌的潜在生物技术应用，将其固定于两种碧根果壳基生物复合材料中，其中孔径更大的碧根果壳生物复合材料展现出更优异的固定效果。