Comparison of gene expression in M. tuberculosis TrxB2-DUC mutant treated or not with atc and DTT

M. tuberculosis thioredoxin reductase (TrxB2) is essential for bacterial survival in vitro and in vivo. To gain insight into the pathways dependent on TrxB2, we compared the mRNA profiles of TrxB2-DUC mutant in the presence and absence of atc and DTT. We grew bacteria in 7H9 medium to an OD of 0.5~0.6. We then added atc to a final concentration of 800 ng/ml and DTT to a final concentration of 2 mM. Samples were collected 24 hr after treatment.

结核分枝杆菌（M. tuberculosis）硫氧还蛋白还原酶（thioredoxin reductase, TrxB2）对细菌的体外与体内存活均至关重要。为深入解析TrxB2依赖的生物学通路，我们对TrxB2-DUC突变株在添加与不添加无水四环素（anhydrotetracycline, atc）及二硫苏糖醇（dithiothreitol, DTT）条件下的mRNA表达谱进行了对比分析。我们将细菌接种于7H9培养基中培养至光密度（optical density, OD）值为0.5~0.6，随后向培养基中加入atc至终浓度800 ng/ml、DTT至终浓度2 mM，并于处理后24小时收集样本。